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KMID : 0604520120380010057
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Journal of the Society of Cosmetic Scientists of Korea
2012 Volume.38 No. 1 p.57 ~ p.65
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Antioxidative Effect and Component Analysis of Eriobotrya japonica Leaf Extracts
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Kim Su-Ji
Park Jin-Oh
Park Soo-Nam
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Abstract
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In the present study, the antioxidative properties, inhibitory activity on tyrosinase, and active components of Eriobotrya japonica (E. japonica) leaf extract were investigated. The free radical (1,1-diphenyl-2-picrylhydrazyl, DPPH) scavenging activity (FSC50) of extract/fraction of E. japonica leaf was in the order 50 % ethanol extract (22.625 μg/mL) < ethyl acetate fraction (6.75) < deglycosylated aglycone fraction (5.06). Reactive oxygen species (ROS) scavenging activities (OSC50) of fraction/extracton ROS generated in Fe3+-EDTA/H2O2 system using the luminol-dependent chemiluminescenceassay were investigated. OSC50 of the ethyl acetate fraction, deglycosylated aglycone fraction, and ethanol extract were 0.75, 0.79, and 1.61 μg/mL, respectively. The cellular protective effects of E. japonica leaf extract on the rose-bengal sensitized photohemolysis of human erythrocytes were investigated. The protective effects of extract/fraction of E. japonica leaf were increased in a in a concentration dependent manner (5 ∼ 50 μg/mL). Especially, τ50 of ethyl acetate fraction at concentrations of 10 μg/mL and 50 μg/mL showed the most protective effects at 390.8 min and 1471.5 min. The inhibitory effect (IC50) on tyrosinase of E. japonica leaf extracts was higher than arbutin, known as a skin-whitening agent. The order of inhibitory effects was acetate fraction (75.25 μg/mL) < 50 % extract (74.1) < deglycosylated aglycone fraction (43.35). TLC of the ethyl acetate fraction showed 7 bands (EJL 1 ∼ EJL 7). HPLC of the aglycone fraction exhibited 2 peaks, kaempferol and quercetin. The amounts of kaempferol and quercetin were 53.7 and 46.3 %. respectively. Therefore, The amounts of kaempferol and its glucoside were a little bit higher than quercetin and its glucoside in E. japonica leaf extract. Accordingly, these findings suggest that extracts/fractions of E. japonica leaf can function as antioxidants in biological systems, especially skin exposed to UV radiation, and protect cellular membranes against ROS. Thus, the extract/fraction of E. japonica leaf may be used in novel functional cosmetics as antioxidants against skin photoaging.
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KEYWORD
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Eriobotrya japonica, skin, antioxidant, component analysis, reactive oxygen species
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